Chemotaxis of Neutrophils

Our body is protected from infectious agents such as toxins and pathogenic micro organisms by the help of a variety of immune cells and molecules. These factors act together and make up the immune system. The inflammatory reaction is one of the most important mechanisms of innate immunity. Once bacteria have invaded the tissue, phagocytes get activated and recruit neutrophils from the blood vessels to the site of infection. This process is a highly regulated sequence of different steps called leukocyte adhesion cascade. When neutrophils have reached the infected tissue, they are guided by a chemotactic gradient released by the bacteria. This movement is called chemotaxis. Finally, the neutrophils catch up the bacteria and engulf them by phagocytosis.    

In this course students learn how to prepare peripheral blood smears in order to visualize and analyse the composition of the different leukocytes. In addition, a chemotaxis transwell assay is applied to demonstrate the migration of neutrophils across an endothelial layer.

Recommended: from grade 9 on
Duration: 1 day
Number of Participants: 20