Transcriptional analysis and RNA processing

RNA plays a major role on the way from gene to protein. Regulation of gene expression can be examined in vitro using RT-PCR, which is a combination of two molecular methods: reverse transcription and polymerase chain reaction.

During transcription a copy of a gene is synthesized to build the messenger RNA (mRNA). This mRNA is used as a template to synthesize a complementary DNA (cDNA) by the reverse transcriptase enzyme. The latter originally derives from retro-viruses. The resulting cDNA can be then detected in a standard PCR.

Regulation of gene expression, structure and function of different RNA types, mRNA maturation and processing belong to the topics presented in this course.

The expression regulation of the galactosidase and actin genes in yeast (Saccharomyces cerevisiae) under different conditions will be analyzed during this course.

The following methods will be carried out:

  • Isolation of RNA from yeast
  • cDNA synthesis by reverse transcription
  • Polymerase chain reaction
  • Agarose gel electrophoresis
  • Microscopy of yeast cells

Duration: 1½  days
Paticipants: max. 20

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